Fabrication of Ultra-Thin Micro-Porous PDMS Membrane for Cell Co-Culture in Blood Brain Barrier Model on Chip

See shorturl.at/jntDN — In vitro human blood-brain barrier (BBB) model is amenable to cellular studies on pathological and drug transport mechanisms related to the central nervous system (CNS). Compared with animal model of human, micro BBB ( μBBB ) based on microfluidic chips is closer to in vivo phenotype and is more reproducible. Porous semipermeable membranes are integral components of the chip model, which provide cellular interfaces and help establish barrier models. This study proposes a convenient and low-cost fabrication method for ultra-thin micro-porous poly(dimethylsiloxane) (PDMS) membrane, which is inspired by paraffin section technique in immunohistochemistry. Microporous PDMS was…