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Application of UV-LEDs for antibiotic resistance genes inactivation – efficiency monitoring with qPCR and transformation

See A dose-response study on a plasmid vector containing ampicillin and kanamycin resistance genes was performed with UVC-LEDs at two different wavelengths (265 and 285 nm) using different UV fluences including 40 mJ/cm2 and 186 cm2, typically associated with 4-log inactivation of bacterial pathogens and viruses, respectively. DNA damage was assessed using qPCR and transformation assays:…

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