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Application of UV-LEDs for antibiotic resistance genes inactivation – efficiency monitoring with qPCR and transformation

See A dose-response study on a plasmid vector containing ampicillin and kanamycin resistance genes was performed with UVC-LEDs at two different wavelengths (265 and 285 nm) using different UV fluences including 40 mJ/cm2 and 186 cm2, typically associated with 4-log inactivation of bacterial pathogens and viruses, respectively. DNA damage was assessed using qPCR and transformation assays: four different qPCR protocols targeting the resistance genes were designed to produce amplicons varying in lengths from 80 to 601 bp to quantify DNA damage. UV irradiation emitted at 265 nm was more efficient than 285 nm, as shown by the transformation and qPCR assays. The inactivation…
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